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iv sze selection  (Sage Science)


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    Sage Science iv sze selection
    Iv Sze Selection, supplied by Sage Science, used in various techniques. Bioz Stars score: 96/100, based on 3518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iv+sze+selection/BluePippin/10__7554_slash_elife__11282-334-43-50
    Average 96 stars, based on 3518 article reviews
    iv sze selection - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Population genomics of intrapatient HIV-1 evolution
    Article Snippet: .. DOI: 10.7554/eLife.11282 16 of 26 (ii) the number of post-tagmentation PCR cycles was raised up to 14 for samples with very low input DNA; (iii) post-PCR purification was done using Qiagen Qiaquick columns to maximize large-inserts throughput as compared to magnetic-bead based protocols; (iv) sze selection was performed using the SageScience BluePippin system with 1.5% agarose gel cassettes and internal marker R2, selecting sizes of 550–900 bp (including dual index Nextera adapters, final insert sizes 400–700 bp); (v) size-selected eluates were pooled, buffer-exchanged into EB (10 mM Tris-HCl), and reconcentrated to at least 2 nM. .. The Illumina MiSeq instrument with 2 2 bp or 2 2 bp sequencing kits (MS-102-2003/MS-1023003) was used to sequence the DNA libraries.

    Purification:

    Article Title: Population genomics of intrapatient HIV-1 evolution
    Article Snippet: .. DOI: 10.7554/eLife.11282 16 of 26 (ii) the number of post-tagmentation PCR cycles was raised up to 14 for samples with very low input DNA; (iii) post-PCR purification was done using Qiagen Qiaquick columns to maximize large-inserts throughput as compared to magnetic-bead based protocols; (iv) sze selection was performed using the SageScience BluePippin system with 1.5% agarose gel cassettes and internal marker R2, selecting sizes of 550–900 bp (including dual index Nextera adapters, final insert sizes 400–700 bp); (v) size-selected eluates were pooled, buffer-exchanged into EB (10 mM Tris-HCl), and reconcentrated to at least 2 nM. .. The Illumina MiSeq instrument with 2 2 bp or 2 2 bp sequencing kits (MS-102-2003/MS-1023003) was used to sequence the DNA libraries.

    Selection:

    Article Title: Population genomics of intrapatient HIV-1 evolution
    Article Snippet: .. DOI: 10.7554/eLife.11282 16 of 26 (ii) the number of post-tagmentation PCR cycles was raised up to 14 for samples with very low input DNA; (iii) post-PCR purification was done using Qiagen Qiaquick columns to maximize large-inserts throughput as compared to magnetic-bead based protocols; (iv) sze selection was performed using the SageScience BluePippin system with 1.5% agarose gel cassettes and internal marker R2, selecting sizes of 550–900 bp (including dual index Nextera adapters, final insert sizes 400–700 bp); (v) size-selected eluates were pooled, buffer-exchanged into EB (10 mM Tris-HCl), and reconcentrated to at least 2 nM. .. The Illumina MiSeq instrument with 2 2 bp or 2 2 bp sequencing kits (MS-102-2003/MS-1023003) was used to sequence the DNA libraries.

    Agarose Gel Electrophoresis:

    Article Title: Population genomics of intrapatient HIV-1 evolution
    Article Snippet: .. DOI: 10.7554/eLife.11282 16 of 26 (ii) the number of post-tagmentation PCR cycles was raised up to 14 for samples with very low input DNA; (iii) post-PCR purification was done using Qiagen Qiaquick columns to maximize large-inserts throughput as compared to magnetic-bead based protocols; (iv) sze selection was performed using the SageScience BluePippin system with 1.5% agarose gel cassettes and internal marker R2, selecting sizes of 550–900 bp (including dual index Nextera adapters, final insert sizes 400–700 bp); (v) size-selected eluates were pooled, buffer-exchanged into EB (10 mM Tris-HCl), and reconcentrated to at least 2 nM. .. The Illumina MiSeq instrument with 2 2 bp or 2 2 bp sequencing kits (MS-102-2003/MS-1023003) was used to sequence the DNA libraries.

    Marker:

    Article Title: Population genomics of intrapatient HIV-1 evolution
    Article Snippet: .. DOI: 10.7554/eLife.11282 16 of 26 (ii) the number of post-tagmentation PCR cycles was raised up to 14 for samples with very low input DNA; (iii) post-PCR purification was done using Qiagen Qiaquick columns to maximize large-inserts throughput as compared to magnetic-bead based protocols; (iv) sze selection was performed using the SageScience BluePippin system with 1.5% agarose gel cassettes and internal marker R2, selecting sizes of 550–900 bp (including dual index Nextera adapters, final insert sizes 400–700 bp); (v) size-selected eluates were pooled, buffer-exchanged into EB (10 mM Tris-HCl), and reconcentrated to at least 2 nM. .. The Illumina MiSeq instrument with 2 2 bp or 2 2 bp sequencing kits (MS-102-2003/MS-1023003) was used to sequence the DNA libraries.



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    iv sze selection  (Sage Science)
    96
    Sage Science iv sze selection
    Iv Sze Selection, supplied by Sage Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iv+sze+selection/BluePippin/10__7554_slash_elife__11282-334-43-50
    Average 96 stars, based on 1 article reviews
    iv sze selection - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

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